What does this strategy enable?
Differential dansylation couples selective chemical tagging with coeluting isotope pairs. Sample-to-reference signal ratios support comparative quantification; labeled standards enable calibrated measurements.
Transferable workflow
Workflow
- 1
Label samples and reference
React compatible metabolites with light or heavy dansyl reagents.
- 2
Mix labeled material
Combine labeled sample with reference material or calibrated standards.
- 3
Separate and measure isotope pairs
Analyze coeluting pairs by LC-MS.
- 4
Calculate comparative abundances
Use pair ratios and appropriate calibration for the intended measurement.
Evidence of reuse
Published implementations and adaptations
These papers applied, adapted, extended, benchmarked or formalized the Strategy after its originating publication. They are examples of precedent, not a ranking of software choices.
Qualitative Metabolome Analysis of Human Cerebrospinal Fluid by 13C-/12C-Isotope Dansylation Labeling Combined with Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Application of isotope dansylation to cerebrospinal-fluid profiling; the sample matrix does not define a new Strategy.
Strategy profile
Components
Sample preparation, Chromatography, Data acquisition, Statistical analysis
Scope and considerations
Designed for
- Comparative profiling of chemically reactive amines and phenols.
Important requirements
- Compatible derivatization chemistry, labeled reference material and LC-MS.
Considerations
- Chemical isotope tags do not measure biological isotope incorporation or metabolic flux.
- Coverage is restricted by reactive functional groups; isotope pairs alone do not confirm structure.
Reusable resources
Publication
Guo K, Li L. Differential 12C-/13C-Isotope Dansylation Labeling and Fast Liquid Chromatography/Mass Spectrometry for Absolute and Relative Quantification of the Metabolome. Analytical Chemistry 81, 3919–3932 (2009).