What does this strategy enable?
Pair rapid chromatographic fraction collection with LC-MS/MS and luminescent bioreporters to connect chemical signals with antibacterial responses.
Transferable workflow
Workflow
- 1
Collect fractions during LC-MS/MS
Spot eluent onto a paper microfluidic device at approximately one fraction per second while acquiring spectra.
- 2
Measure reporter responses
Expose fractions to luminescent bioreporters and image the response.
- 3
Align activity with chemistry
Match reporter signals to chromatographic profiles to prioritize active compounds.
Strategy profile
Related objectives
Phenotype association
Components
Chromatography, Data acquisition, Biological validation
Scope and considerations
Designed for
- Antibacterial activity screening of extracts or compound mixtures.
Important requirements
- Custom rapid fractionation hardware and compatible paper devices.
- LC-MS/MS, luminescent bioreporters and response imaging.
- Reliable correspondence between fraction positions and retention times.
Considerations
- Reporter responses are assay-specific; confirm prioritized activity with follow-up experiments.
- Coelution can complicate assigning activity to one compound.
Reusable resources
✓ Published method available
Publication
Geibel C, et al. Analytical Chemistry 97, 24093-24104 (2025).