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Isotope tracing and flux analysis

FAMetA

Estimate fatty-acid synthesis, import, elongation and desaturation from carbon-13 isotopologue profiles.

LC-MSR packageOpen source
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Overview

FAMetA is an R package for model-based fatty-acid metabolic analysis. The publication also describes a web application. Its workflow uses LipidMS for preprocessing and accucor for natural-abundance correction before fitting fatty-acid metabolic contributions.

What can this tool do?

Metabolic-flux estimation

Common uses

  • Investigate fatty-acid metabolic contributions in tracer experiments.

Scientific fit

Primary function

Isotope tracing and flux analysis

Data compatibility

Inputs

Isotope-tracing data

Outputs

Statistical results, Human-readable report

Input formats

Fatty-acid carbon-13 mass-isotopologue profiles prepared for the R workflow

Output formats

Fitted fatty-acid metabolic contributions and graphical summaries

Scope and considerations

Designed for

  • Model-based analysis of fatty-acid labeling.

Not designed for

  • Untargeted structure identification or unrestricted whole-network flux analysis.

Important requirements

  • Suitable carbon-13 labeling data and the R/LipidMS/accucor dependencies.

Considerations

  • Model assumptions, tracer choice and isotopologue measurement quality constrain interpretation.

Access and documentation

Primary access

License declared as GPL (>= 2) in package DESCRIPTION. The paper describes a web app, but current hosted availability was not verified.

License: GPL-2.0-or-later

Evidence and review

Discovered through the Enveda review intake; official repository and primary publication metadata checked on 2026-10-02. Editorial review only; software and performance not execution-tested.

Core publications

Resource papers, direct updates and tool-specific methods. Selection policy.

  1. FAMetA: a mass isotopologue-based tool for the comprehensive analysis of fatty acid metabolism. Briefings in Bioinformatics (2023). doi:10.1093/bib/bbad064

Related tools

13C-SpaceM analysis codeSimilar function · Analyze spatial carbon-13 labeling of fatty acids in single cells and tissue imaging experiments.AerithSimilar function · An R package for reading, annotating, scoring, and visualizing isotopic mass spectra in stable-isotope-probing workflows for metabolites and peptides.IsoPairFinderSimilar function · An R package for identifying biologically relevant 12C/13C feature pairs in stable-isotope-tracing metabolomics, including differential analysis and redundant-feature merging.isoSCANSimilar function · Integrate stable-isotope-labeling peaks from GC chemical-ionization mass spectrometry data.

Entry history

Changes to this catalogue entry, not the tool's software release history. Acceptance and editorial review are separate.

  1. 2026-10-02 · First committed entry

    Add five source-verified isotope and spatial tools from Enveda intake

    View change · Entry at this revision

Full GitHub history