Pathway and biological interpretation

mummichog

Generate pathway and network hypotheses from untargeted metabolomics feature tables.

LC-MSPython packageCommand lineSource available with restrictions

Overview

mummichog tests patterns of candidate metabolite matches across metabolic networks, allowing functional exploration before every feature has an identification.

What can this tool do?

Pathway enrichment

Common uses

  • Prioritize pathways associated with differential features.
  • Explore a feature table with a species-appropriate metabolic model.

Scientific fit

Primary function

Pathway and biological interpretation

Data compatibility

Inputs

Feature or peak table, Sample metadata

Outputs

Statistical results, Human-readable report

Input formats

Other, Feature table with m/z and statistical results

Output formats

Other, Pathway enrichment and network results

Scope and considerations

Designed for

  • Prioritize pathways associated with differential features.

Not designed for

  • Confirming individual metabolite identities or measuring metabolic flux.

Important requirements

  • Feature masses, statistical results, acquisition information and a suitable metabolic model.

Considerations

  • Background features and adduct assumptions affect enrichment. The current repository labels version 3 as development and retains a version 2 branch.

Access and documentation

Public source code; pin the intended release branch and consult its license.

Evidence and review

Documentation-based editorial review on 7 September 2026. Scope and limitations summarize the linked official sources; software execution and developer verification were not assessed.

Core publications

Resource papers, direct updates and tool-specific methods. Selection policy.

  1. Li S, Park Y, Duraisingham S, et al. Predicting network activity from high throughput metabolomics. PLoS Comput Biol 9, e1003123 (2013). doi:10.1371/journal.pcbi.1003123

Related tools

Entry history

Changes to this catalogue entry, not the tool's software release history. Acceptance and editorial review are separate.

  1. 2026-09-08 · Entry update

    Audit core publications across existing tools

    View change · Entry at this revision
  2. 2026-09-07 · First committed entry

    Add 50 documented metabolomics resources

    View change · Entry at this revision

Full GitHub history