Overview
MBROLE3 tests overrepresentation of chemical and functional annotations, with identifier conversion and separate direct and indirect annotation results.
Also known as: MBROLE, MBROLE 3
What can this tool do?
Common uses
- Test functional enrichment in lists of annotated metabolites.
Scientific fit
Primary function
Pathway and biological interpretation
Data compatibility
Inputs
Compound list or annotations
Outputs
Statistical results, Human-readable report
Input formats
Compound IDs separated by tabs or newlines; optional background list
Output formats
Enrichment tables, TSV download and dot/bar plots
Scope and considerations
Designed for
- MBROLE3 tests overrepresentation of chemical and functional annotations, with identifier conversion and separate direct and indirect annotation results.
Not designed for
- Identification of unknown spectra or direct testing of unannotated m/z features.
Important requirements
- Mapped compound identifiers, selected annotation vocabularies and a suitable background set.
Considerations
- Use a background reflecting measurable compounds. Indirect literature/protein associations are not direct experimental evidence of pathway activity.
Access and documentation
The primary paper describes a free service without login. No software license or service execution validation is asserted.
Evidence and review
Enveda intake checked against official documentation, license information and primary DOI/title/year on 2026-10-22. Editorial review only; no execution or independent benchmark.
Core publications
Resource papers, direct updates and tool-specific methods. Selection policy.
- MBROLE3: improved functional enrichment of chemical compounds for metabolomics data analysis. Nucleic Acids Research (2023). doi:10.1093/nar/gkad405
Related tools
Entry history
Changes to this catalogue entry, not the tool's software release history. Acceptance and editorial review are separate.
- 2026-10-23 · First committed entry
Add ten reviewed Enveda tools with input and applicability limits
View change · Entry at this revision